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protein 4  (ABclonal Biotechnology)
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ABclonal Biotechnology protein 4
Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Protein 4, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti timd4  (Atlas Antibodies)
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Atlas Antibodies anti timd4
Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Anti Timd4, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+timd4+antibody/Anti-TIMD4/bio_rxiv__64898__2026__03__16__712250-61-17-28
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anti timd4 - by Bioz Stars, 2026-11
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dr s hartle  (Atlas Antibodies)
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Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Dr S Hartle, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dr s hartle - by Bioz Stars, 2026-11
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antibodies against timd 4  (Proteintech)
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Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
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antibodies against timd 4 - by Bioz Stars, 2026-11
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Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Primary Antibodies Against Tim 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+timd4+antibody/TIMD4+Antibody/pm41064000-108-26-31
Average 94 stars, based on 1 article reviews
primary antibodies against tim 4 - by Bioz Stars, 2026-11
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tim 4  (Proteintech)
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Proteintech tim 4
Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Tim 4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+timd4+antibody/TIMD4+Antibody/pm41064000-116-15-23
Average 94 stars, based on 1 article reviews
tim 4 - by Bioz Stars, 2026-11
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tim4  (Proteintech)
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Proteintech tim4
Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of <t>TIMD4.</t> (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.
Tim4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+timd4+antibody/TIMD4+Antibody/pmc12354832__41467_2025_62690_MOESM2_ESM-70-123-131
Average 94 stars, based on 1 article reviews
tim4 - by Bioz Stars, 2026-11
94/100 stars
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Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of TIMD4. (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.

Journal: International Journal of Molecular Medicine

Article Title: Hypothermic machine perfusion protects DCD graft liver from ischemia-reperfusion injury by enhancing macrophage efferocytosis via KLF2-NLRP3 signaling

doi: 10.3892/ijmm.2026.5756

Figure Lengend Snippet: Upregulation of the endothelial cell protective molecule KLF2 promotes macrophage efferocytosis. (A) In vivo assessment and (B) quantification of efferocytosis (proportion of CD68-positive cells engulfing TUNEL-positive apoptotic cells relative to the total CD68-positive cell population). Arrows represent macrophage efferocytosis events. (C) Schematic diagram of the co-culture protocol for HUVECs with varying KLF2 expression levels and macrophages. (D) Macrophages (CMTPX + ) were co-cultured with apoptotic Jurkat cells (CMFDA + ) for 45 min, and efferocytosis was quantitatively assessed in vitro by fluorescence co-localization analysis. (E) Statistical analysis of fluorescence signals from HUVECS of ov-control and ov-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (F) Statistical analysis of fluorescence signals from HUVECS of sh-control and sh-KLF2 groups was performed using Image-Pro Plus 6.0 software (n=3). (G) Representative staining of TIMD4. (H) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0. (I) Immunofluorescence of TIMD4 from HUVECS of ov-control and ov-KLF2 groups was quantified using Image-pro Plus 6.0 (n=3). * P<0.05, ** P<0.01, *** P<0.001. HMP, hypothermic machine perfusion; KLF2, Kruppel-like Factor 2; AC, apoptotic Jurkat cell; HUVEC, human umbilical vein endothelial cells; CMTPX, CellTracker™ Red CMTPX dye; CMFDA, 5-Chloromethylfluorescein Diacetate; CS, cold storage; Rep, reperfusion; TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4; ov, overexpression; sh, short hairpin.

Article Snippet: The primary antibodies were as follows: Anti-caspase 3 (1:200; cat. no. 9661, Cell Signaling Technology, Inc.), anti-myeloperoxidase (MPO; 1:1,000; cat. no. ab208670, Abcam), anti-KLF2 (1:200; cat. no. bs-2772R, BIOSS) and anti-T cell immunoglobulin and mucin domain-containing protein 4 (TIMD4; 1:200; cat. no. a17807, ABclonal Biotech Co., Ltd.).

Techniques: In Vivo, TUNEL Assay, Co-Culture Assay, Expressing, Cell Culture, In Vitro, Fluorescence, Control, Software, Staining, Immunofluorescence, Over Expression